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Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
Expression
RNA: detected in 8 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045702
Standard validation
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417530)
Target mass (kDa)
21.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA045702
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
