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ANTIBODY INFORMATION
 
 

Antibody HPA059627

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA059627  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA059627

  
Immunocytochemistry

Supported  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA059627

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.  

Antibody dilution

 1:58  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA059627

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 10 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA059627

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 33.8, 33.4, 32.6, 31.3, 28.9  

Antibody dilution

 1:190  

PROTEIN ARRAY
 
 

Antibody HPA059627

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2300  

ANTIGEN INFORMATION
 
 

Antibody HPA059627

  

Antigen

 Recombinant protein fragment  

Length (aa)

 62  

Antigen sequence

 
ADRSMEKKKGEPRTRAEARPWVDEDLKDSSDLHQAEEDADEWQESEENVE
HIPFSHNHYPEK
 

Matching transcripts

 IYD-001 - ENSP00000343763 [100%]
IYD-002 - ENSP00000229447 [100%]
IYD-004 - ENSP00000376085 [100%]
IYD-005 - ENSP00000441276 [100%]
IYD-006 - ENSP00000376084 [100%]
 

Other gene match

  
ANTIGEN VIEW
IYD-001
IYD-002
IYD-004
IYD-005
IYD-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.