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UQCRC1

ANTIBODY INFORMATION
 
 

Antibody HPA002815

  

Antibody HPA003525

  

Antibody CAB033782

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA002815   HPA003525   sc-65238  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 3   3   12  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA002815

  

Antibody HPA003525

  

Antibody CAB033782

  
Immunocytochemistry

Supported      
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002815

  

Antibody HPA003525

  

Antibody CAB033782

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.      

Antibody dilution

 1:45      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002815

  

Antibody HPA003525

  

Antibody CAB033782

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA003525 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA002815 across 72 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in granular pattern in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:150   1:600  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002815

  

Antibody HPA003525

  

Antibody CAB033782

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 52.6   52.6   52.6  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002815

  

Antibody HPA003525

  

Antibody CAB033782

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA002815

  

Antibody HPA003525

  

Antibody CAB033782

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 105   137    

Antigen sequence

 
GDIVQNCSLEDSQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPL
AQAVEGPSENVRKLSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQ
KHLGG
  
DDALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPL
ASGAVANKLCQSFQTFSICYAETGLLGAHFVCDRMKIDDMMFVLQGQWMR
LCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGR
  

											 
																				

																		

										
											
Matching transcripts

												
																					UQCRC1-001 - ENSP00000203407 [100%] 

											 
																						UQCRC1-001 - ENSP00000203407 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
UQCRC1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002815

												
										 
																				
											
											
											
Antibody HPA003525

												
										 
																				
											
											
											
Antibody CAB033782

												
										 
																		

																	

										
											
1.

												
																						Integration of cardiac proteome biology and medicine by a specialized knowledgebase

	Zong NC et al

	Circ Res 2013;113(9):1043-53

			Application: IHC				
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization

	Desmurs M et al

	Mol Cell Biol 2015;35(7):1139-56

			Application: WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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