We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line RT4 shows localization to focal adhesion sites.
Antibody dilution
1:58
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA049926
Antibody HPA053406
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human duodenum shows strong nuclear membranous positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 66 cell types
RNA: detected in 35 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:75
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049926
Antibody HPA053406
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
126.4, 54.4, 46
126.4, 85.5, 82.7
Antibody dilution
1:290
1:240
PROTEIN ARRAY
Antibody HPA049926
Antibody HPA053406
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
