WIZ - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

WIZ

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA022923

Antibody HPA023774

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA022923

HPA023774

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA022923

Antibody HPA023774

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA022923

Antibody HPA023774

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA023774.

Validated

Antibody staining overlaps with antibody HPA022923.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.

Antibody dilution

1:100

1:27

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA022923

Antibody HPA023774

Standard validation

Supported

Figure description

Immunohistochemical staining of human pancreas shows strong nuclear and slightly weaker nuclear positivity in exocrine as well as endocrine cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 78 cell types

Retrieval

HIER pH6

Antibody dilution

1:85

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA022923

Antibody HPA023774

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

103.3, 103, 89.4, 89.1, 85.4

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA022923

Antibody HPA023774

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA022923

Antibody HPA023774

Antigen

Recombinant protein fragment

Length (aa)

123

Antigen sequence

SPSDLHISPLAKKLPPPPGSPLGHSPTASPPPTARKMFPGLAAPSLPKKL
KPEQIRVEIKREMLPGALHGELHPSEGPWGAPREDMTPLNLSSR

LKKKSKPCLIKKEPPAGDLAPALAEDGPPTVAPGPVQSPLPLSPLAGRPG
KPGAGPAQVPRELSLTPITGAKPSATGYLGSVAAKRPLQEDRLLPAEVKA
KTYIQTELPFKAKTLHEKTSHSS

Matching transcripts

WIZ-001 - ENSP00000263381 [100%]
WIZ-002 - ENSP00000469022 [100%]
WIZ-006 - ENSP00000469534 [100%]
WIZ-201 - ENSP00000373933 [100%]
WIZ-202 - ENSP00000445824 [100%]

Other gene match

ANTIGEN VIEW

WIZ-001	WIZ-002	WIZ-006	WIZ-201	WIZ-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.