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CD22

ANTIBODY INFORMATION
 
 

Antibody HPA024353

  

Antibody CAB002418

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA024353   NCL-CD22-2  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 6   1  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA024353

  

Antibody CAB002418

  
Immunohistochemistry

Supported  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024353

  

Antibody CAB002418

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in a subset of lymphoid cells.   Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.  

Expression

 RNA: detected in 30 tissues
Protein: detected in 16 cell types		   RNA: detected in 30 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1200   1:30  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024353

  

Antibody CAB002418

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 95.3, 85.7, 84.5, 75.8, 73.2, 26   95.3, 85.7, 84.5, 75.8, 75.4, 73.2, 26, 18.9, 18.4, 16.9, 16.7, 16.1, 15.5, 12.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024353

  

Antibody CAB002418

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA024353

  

Antibody CAB002418

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 127    

Antigen sequence

 
DLESFILFHNPEYNKNTSKFDGTRLYESTKDGKVPSEQKRVQFLGDKNKN
CTLSIHPVHLNDSGQLGLRMESKTEKWMERIHLNVSERPFPPHIQLPPEI
QESQEVTLTCLLNFSCYGYPIQLQWLL
   

Matching transcripts

 CD22-001 - ENSP00000442279 [100%]
CD22-002 - ENSP00000085219 [100%]
CD22-003 - ENSP00000441237 [100%]
CD22-004 - ENSP00000469984 [100%]
CD22-007 - ENSP00000471972 [100%]
CD22-201 - ENSP00000270311 [100%]
CD22-202 - ENSP00000339349 [100%]
   

Other gene match

    
ANTIGEN VIEW
CD22-001
CD22-002
CD22-003
CD22-004
CD22-007
CD22-201
CD22-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.