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ANTIBODY INFORMATION
 
 

Antibody HPA057177

  

Antibody HPA059976

  

Antibody CAB008646

  

Antibody CAB016201

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   AbFrontier
   Santa Cruz Biotechnology
  

Product name

 HPA057177   HPA059976   LF-PA0109   sc-53498  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Ammonium Sulfate Precipitation   Protein A/G  

Other gene match

        

Released in version

 13   13   13   13  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA057177

  

Antibody HPA059976

  

Antibody CAB008646

  

Antibody CAB016201

  
Immunohistochemistry

Supported   Supported  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA057177

  

Antibody HPA059976

  

Antibody CAB008646

  

Antibody CAB016201

  

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

   
 

 

    

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human lactating breast shows plasma positivity.   Immunohistochemical staining of human lactating breast shows moderate membranous positivity with additional plasma positivity.   Immunohistochemical staining of human lactating breast shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human lactating breast shows moderate membranous positivity with additional plasma positivity.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 1 cell types		   RNA: detected in 33 tissues
Protein: detected in 8 cell types		   RNA: detected in 33 tissues
Protein: detected in 10 cell types		   RNA: detected in 33 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:3000   1:20000   1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA057177

  

Antibody HPA059976

  

Antibody CAB008646

  

Antibody CAB016201

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		        

Target mass (kDa)

 78.2, 78, 76.6, 73.2   78.2, 78, 76.6, 73.2   78.2, 78, 76.6, 73.2, 20.3   78.2, 78, 76.6, 73.2, 20.3  

Antibody dilution

 1:80   1:300   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA057177

  

Antibody HPA059976

  

Antibody CAB008646

  

Antibody CAB016201

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500   1:3200      

ANTIGEN INFORMATION
 
 

Antibody HPA057177

  

Antibody HPA059976

  

Antibody CAB008646

  

Antibody CAB016201

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Not known  

Length (aa)

 37   62      

Antigen sequence

 
RVVWCAVGEQELRKCNQWSGLSEGSVTCSSASTTEDC
  
PNHAVVSRMDKVERLKQVLLHQQAKFGRNGSDCPDKFCLFQSETKNLLFN
DNTECLARLHGK
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					LTF-001 - ENSP00000231751 [100%] 
LTF-002 - ENSP00000397427 [100%] 
LTF-003 - ENSP00000405546 [100%] 
LTF-011 - ENSP00000405719 [100%] 

											 
																						LTF-001 - ENSP00000231751 [100%] 
LTF-002 - ENSP00000397427 [100%] 
LTF-003 - ENSP00000405546 [100%] 
LTF-011 - ENSP00000405719 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LTF-001
LTF-002
LTF-003
LTF-011
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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