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Antibody staining overlaps with antibody HPA054197.
Validated
Antibody staining overlaps with antibody HPA047752.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
Antibody dilution
1:192
1:72
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA047752
Antibody HPA054197
Antibody CAB017042
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
Expression
RNA: detected in 37 tissues Protein: detected in 34 cell types
RNA: detected in 37 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:4000
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047752
Antibody HPA054197
Antibody CAB017042
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
38.4, 35.3
38.4, 35.3, 30.7
38.4, 35.3, 30.7, 9.6
Antibody dilution
1:960
1:360
1:500
PROTEIN ARRAY
Antibody HPA047752
Antibody HPA054197
Antibody CAB017042
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
