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CLDN11

ANTIBODY INFORMATION
 
 

Antibody HPA013166

  

Antibody CAB009789

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA013166   sc-25711  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   3  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA013166

  

Antibody CAB009789

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA013166

  

Antibody CAB009789

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human hippocampus shows cytoplasmic positivity in neuropil.   Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic positivity in glial cells and neuropil.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 16 cell types		   RNA: detected in 35 tissues
Protein: detected in 52 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:175  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA013166

  

Antibody CAB009789

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 22   22, 18  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA013166

  

Antibody CAB009789

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA013166

  

Antibody CAB009789

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 27    

Antigen sequence

 
DAQAFGENRFYYTAGSSSPTHAKSAHV
   

Matching transcripts

 CLDN11-001 - ENSP00000064724 [100%]
   

Other gene match

    
ANTIGEN VIEW
CLDN11-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA013166

  

Antibody CAB009789

  

1.

 Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts
Marthandan S et al
Biomed Res Int 2015;2015:731938
Application: WB 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.