COX15 - Antibodies - The Human Protein Atlas

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COX15

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA037727

Antibody HPA037728

Antibody HPA066096

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA037727

HPA037728

HPA066096

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA037727

Antibody HPA037728

Antibody HPA066096

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA037727

Antibody HPA037728

Antibody HPA066096

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A549 shows localization to mitochondria.

Antibody dilution

1:58

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA037727

Antibody HPA037728

Antibody HPA066096

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 57 cell types

RNA: detected in 37 tissues
Protein: detected in 26 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:20

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA037727

Antibody HPA037728

Antibody HPA066096

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

46, 43.8

Antibody dilution

1:250

1:190

PROTEIN ARRAY

Antibody HPA037727

Antibody HPA037728

Antibody HPA066096

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2350

ANTIGEN INFORMATION

Antibody HPA037727

Antibody HPA037728

Antibody HPA066096

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

LTESGLSMVDWHLIKEMKPPTSQEEWEAEFQRYQQFPEFKILNHDMTLTE
FKFI

MQRLLFPPLRALKGRQYLPLLAPRAAPRAQCDCIRRPLRPGQYSTISEVA
LQSGR

MGESWIPEDLFTFSPILRNVFENPTMVQFD

Matching transcripts

COX15-001 - ENSP00000016171 [100%]
COX15-002 - ENSP00000359514 [100%]

Other gene match

ANTIGEN VIEW

COX15-001	COX15-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.