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CD74

ANTIBODY INFORMATION
 
 

Antibody HPA010592

  

Antibody CAB002506

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA010592   NCL-LN2  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 3   1  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA010592

  

Antibody CAB002506

  
Immunocytochemistry

  Supported  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA010592

  

Antibody CAB002506

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.  

Antibody dilution

   1:154  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010592

  

Antibody CAB002506

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.   Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells  

Expression

 RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:160  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010592

  

Antibody CAB002506

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 35, 33.5, 26.4   35, 33.5, 26.4, 18.3, 17.2  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA010592

  

Antibody CAB002506

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA010592

  

Antibody CAB002506

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 108    

Antigen sequence

 
PKPVSKMRMATPLLMQALPMGALPQGPMQNATKYGNMTEDHVMHLLQNAD
PLKVYPPLKGSFPENLRHLKNTMETIDWKVFESWMHHWLLFEMSRHSLEQ
KPTDAPPK
   

Matching transcripts

 CD74-001 - ENSP00000230685 [100%]
CD74-003 - ENSP00000009530 [100%]
CD74-004 - ENSP00000430614 [100%]
   

Other gene match

    
ANTIGEN VIEW
CD74-001
CD74-003
CD74-004

RELEVANT PUBLICATIONS
 
 

Antibody HPA010592

  

Antibody CAB002506

  

1.

 Expression of RCAS1 protein in microglia/macrophages accompanying brain tumours. An immunofluorescence study
Adamek D et al
Folia Neuropathol 2009;47(3):240-6
    

2.

 CD74: a new prognostic factor for patients with malignant pleural mesothelioma
Otterstrom C et al
Br J Cancer 2014;110(8):2040-6
Application: IHC 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.