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ZFP64

ANTIBODY INFORMATION
 
 

Antibody HPA035112

  

Antibody HPA058387

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035112   HPA058387  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA035112

  

Antibody HPA058387

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035112

  

Antibody HPA058387

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.  

Antibody dilution

   1:41  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035112

  

Antibody HPA058387

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human liver shows nuclear positivity in hepatocytes.   Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 4 cell types		   RNA: detected in 37 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:30  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035112

  

Antibody HPA058387

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 74.6, 74.5, 68.6   74.6, 74.5, 68.6  

Antibody dilution

 1:250   1:140  

PROTEIN ARRAY
 
 

Antibody HPA035112

  

Antibody HPA058387

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1650  

ANTIGEN INFORMATION
 
 

Antibody HPA035112

  

Antibody HPA058387

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   88  

Antigen sequence

 
SFDTKQPSNLSKHMKKFHGDMVKTEALERKDTGRQSSRQVAKLDAKKSFH
CDICDASFMREDSLRSHKRQHSEYSESKNSDVTVLQFQIEPS
  
SEGRVKIIVGHQVPQANTIVQAAAAAVNIVPPALVAQNPEELPGNSRLQI
LRQVSLIAPPQSSRCPSEAGAMTQPAVLLTTHEQTDGA
 

Matching transcripts

 ZFP64-003 - ENSP00000216923 [99%]
ZFP64-005 - ENSP00000344615 [99%]
ZFP64-008 - ENSP00000360570 [99%]
  ZFP64-003 - ENSP00000216923 [100%]
ZFP64-005 - ENSP00000344615 [100%]
ZFP64-008 - ENSP00000360570 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZFP64-003
ZFP64-005
ZFP64-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.