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SNX1

ANTIBODY INFORMATION
 
 

Antibody HPA047373

  

Antibody HPA052761

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047373   HPA052761  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA047373

  

Antibody HPA052761

  
Immunocytochemistry


Validated

Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot


Validated
   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047373

  

Antibody HPA052761

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Whole cells of segmented cells in 10x-images    

Antibody dilution

 1:30    
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA052761.		  
Validated

Antibody staining overlaps with antibody HPA047373.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line HeLa shows localization to endosomes & lysosomes.   Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.  

Antibody dilution

 1:30   1:38  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047373

  

Antibody HPA052761

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:75    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA047373

  

Antibody HPA052761

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 63.1, 59.1, 51.8, 13.6, 11.1    

Loading control

 Total protein image    

Antibody dilution

 1:67    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 63.1, 59.1, 51.8, 13.6, 11.1   63.1, 59.1  

Antibody dilution

 1:150   1:190  

PROTEIN ARRAY
 
 

Antibody HPA047373

  

Antibody HPA052761

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1200   1:1500  

ANTIGEN INFORMATION
 
 

Antibody HPA047373

  

Antibody HPA052761

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 65   64  

Antigen sequence

 
GGGGCSASERLPPPFPGLEPESEGAAGGSEPEAGDSDTEGEDIFTGAAVV
SKHQSPKITTSLLPI
  
NNGSKENGIHEEQDQEPQDLFADATVELSLDSTQNNQKKVLAKTLISLPP
QEATNSSKPQPTYE
 

Matching transcripts

 SNX1-001 - ENSP00000453785 [100%]
SNX1-002 - ENSP00000453567 [100%]
SNX1-003 - ENSP00000261889 [100%]
SNX1-201 - ENSP00000486714 [100%]
SNX1-009 - ENSP00000452668 [98%]
  SNX1-001 - ENSP00000453785 [100%]
SNX1-003 - ENSP00000261889 [100%]
 

Other gene match

    
ANTIGEN VIEW
SNX1-001
SNX1-002
SNX1-003
SNX1-009
SNX1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.