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ANTIBODY INFORMATION
 
 

Antibody HPA004842

  

Antibody HPA056953

  

Antibody CAB016057

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA004842   HPA056953   sc-12733  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 5   12   4  

References

   1    

VALIDATION SUMMARY
 
 

Antibody HPA004842

  

Antibody HPA056953

  

Antibody CAB016057

  
Immunohistochemistry

Supported   Supported  

Validated
  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004842

  

Antibody HPA056953

  

Antibody CAB016057

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 41 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human bone marrow shows strong positivity in erythrocytes and subsets of hematopoietic cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.   Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in erythrocytes.  

Expression

 RNA: detected in 16 tissues
Protein: detected in 34 cell types		   RNA: detected in 16 tissues
Protein: detected in 3 cell types		   RNA: detected in 16 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:1500   1:20  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004842

  

Antibody HPA056953

  

Antibody CAB016057

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 17.9, 17.6   208.2, 206.3, 206, 115.8   208.2, 206.3, 206, 115.8, 17.9, 17.6, 15, 12.9, 12.5  

Antibody dilution

 1:250   1:1750   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004842

  

Antibody HPA056953

  

Antibody CAB016057

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:22100    

ANTIGEN INFORMATION
 
 

Antibody HPA004842

  

Antibody HPA056953

  

Antibody CAB016057

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 129   100    

Antigen sequence

 
QNVMHIVRGSLCFVLKHIHQELDKELGESEGLSDDEETISTRVVRRRVFL
KGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHE
EVELRGSGLQPDLIEGRKGAQIVKRASLK
  
FVLVSDKHRMSFPETVDEILDVSEDEGEELISFKAERRDSRDVDEEKELL
DFVPKLDQVVESPAIPRIPCAMPETVVIRSEEQEQASKEYDEDSLIPSSP
  

											 
																				

																		

										
											
Matching transcripts

												
																					ANK1-003 - ENSP00000319123 [100%] 
ANK1-009 - ENSP00000430368 [81%] 

											 
																						ANK1-001 - ENSP00000339620 [100%] 
ANK1-002 - ENSP00000289734 [100%] 
ANK1-006 - ENSP00000265709 [93%] 
ANK1-007 - ENSP00000430174 [93%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ANK1-001
ANK1-002
ANK1-003
ANK1-006
ANK1-007
ANK1-009
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA004842

												
										 
																				
											
											
											
Antibody HPA056953

												
										 
																				
											
											
											
Antibody CAB016057

												
										 
																		

																	

										
											
1.

												
																					
											 
																							The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration

	Hall AE et al

	Cell Death Dis 2016;7:e2184

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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