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EDC4

ANTIBODY INFORMATION
 
 

Antibody HPA041164

  

Antibody HPA043038

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041164   HPA043038  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041164

  

Antibody HPA043038

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Supported    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041164

  

Antibody HPA043038

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytoplasmic bodies.   Immunofluorescent staining of human cell line HEK 293 shows localization to cytoplasmic bodies.  

Antibody dilution

 1:34   1:8  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041164

  

Antibody HPA043038

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human colon shows strong ctoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 68 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:40    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA041164

  

Antibody HPA043038

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 151.7   151.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041164

  

Antibody HPA043038

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA041164

  

Antibody HPA043038

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 108   108  

Antigen sequence

 
VERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRL
ERSIRDEIKKTVPPCVSRSLEPMAGQLSNSVATKLTAVEGSMKENISKLL
KSKNLTDA
  
QRKVLYVMELLQNQEEGHACFSSISEFLLTHPVLSFGIQVVSRCRLRHTE
VLPAEEENDSLGADGTHGAGAMESAAGVLIKLFCVHTKALQDVQIRFQPQ
LNPDVVAP
 

Matching transcripts

 EDC4-001 - ENSP00000351811 [100%]
  EDC4-001 - ENSP00000351811 [100%]
 

Other gene match

    
ANTIGEN VIEW
EDC4-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.