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MYO16

ANTIBODY INFORMATION
 
 

Antibody HPA040110

  

Antibody HPA058769

  

Antibody HPA071948

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040110   HPA058769   HPA071948  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 8   16   16  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA040110

  

Antibody HPA058769

  

Antibody HPA071948

  
Immunocytochemistry

  Approved   Approved  
Immunohistochemistry

Uncertain      
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040110

  

Antibody HPA058769

  

Antibody HPA071948

  

Standard validation

   Approved   Approved  

     

Figure description

   Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.   Immunofluorescent staining of human cell line SH-SY5Y shows localization to nuclear membrane & vesicles.  

Antibody dilution

   1:80   1:51  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040110

  

Antibody HPA058769

  

Antibody HPA071948

  

Standard validation

 Uncertain      

     

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.      

Expression

 RNA: detected in 2 tissues
Protein: detected in 25 cell types		      

Retrieval

 HIER pH6      

Antibody dilution

 1:250      

Literature conformity

 No avaliable gene/protein characterization data.      

RNA consistency

 Not consistent with RNA expression data.      

WESTERN BLOT
 
 

Antibody HPA040110

  

Antibody HPA058769

  

Antibody HPA071948

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 208.8, 206.1, 106.2   208.8, 206.1, 106.2   208.8, 206.1  

Antibody dilution

 1:250   1:270   1:170  

PROTEIN ARRAY
 
 

Antibody HPA040110

  

Antibody HPA058769

  

Antibody HPA071948

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:12000   1:3200   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA040110

  

Antibody HPA058769

  

Antibody HPA071948

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 81   91   81  

Antigen sequence

 
EGVTMETAYSPGNQNGVLDFFFQKPSGFLTLLDEESQMIWSVESNFPKKL
QSLLESSNTNAVYSPMKDGNGNVALKDHGTA
  
LSASTLAQEEPYEEIIHDLPVLSSKLSPLVLPIAKQDSLLEKDIMFKDAT
KGLCKQQSQDSIPENPMMSGSTKPEQVKLMPPAPNDDLATL
  
LFQSKLSQTGSLVSAYPSFKFRGHKSALLSKKMTASSIIGENKNYLELSK
LLKKKGTSTFLQRLERGDPVTIASQLRKSLM
 

Matching transcripts

 MYO16-001 - ENSP00000350160 [100%]
MYO16-002 - ENSP00000251041 [100%]
MYO16-201 - ENSP00000349145 [100%]
MYO16-202 - ENSP00000401633 [100%]
  MYO16-001 - ENSP00000350160 [100%]
MYO16-002 - ENSP00000251041 [100%]
MYO16-201 - ENSP00000349145 [100%]
MYO16-202 - ENSP00000401633 [100%]
  MYO16-001 - ENSP00000350160 [100%]
MYO16-201 - ENSP00000349145 [100%]
MYO16-202 - ENSP00000401633 [100%]
 

Other gene match

      
ANTIGEN VIEW
MYO16-001
MYO16-002
MYO16-201
MYO16-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.