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CTNNA1

ANTIBODY INFORMATION
 
 

Antibody HPA046119

  

Antibody HPA063535

  

Antibody CAB021089

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   GenWay Biotech
  

Product name

 HPA046119   HPA063535   15-288-22836  

Host species

 Rabbit   Rabbit   Hen  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 12   14   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA046119

  

Antibody HPA063535

  

Antibody CAB021089

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Approved     Approved  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046119

  

Antibody HPA063535

  

Antibody CAB021089

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane & cell junctions.   Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane & cell junctions.    

Antibody dilution

 1:21   1:32    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046119

  

Antibody HPA063535

  

Antibody CAB021089

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.     Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 74 cell types		     RNA: detected in 37 tissues
Protein: detected in 50 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:20     1:850  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046119

  

Antibody HPA063535

  

Antibody CAB021089

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 100.1, 92.7, 23.7, 17.5   100.1, 92.7   100.1, 92.7, 59.5, 23.7, 17.5, 17.1, 15.2, 14.9, 14.5, 13.4, 13.3, 12.9, 12.2, 11.6, 11.2, 10.4, 8.6, 8.1, 5.8, 4.7, 4.5, 4.3, 3.8, 2.5  

Antibody dilution

 1:250   1:110   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA046119

  

Antibody HPA063535

  

Antibody CAB021089

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1150    

ANTIGEN INFORMATION
 
 

Antibody HPA046119

  

Antibody HPA063535

  

Antibody CAB021089

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 53   42    

Antigen sequence

 
LADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIM
AAK
  
TASDDASQHQGGGGGELAYALNNFDKQIIVDPLSFSEERFRP
  

											 
																				

																		

										
											
Matching transcripts

												
																					CTNNA1-001 - ENSP00000304669 [100%] 
CTNNA1-006 - ENSP00000427821 [100%] 
CTNNA1-011 - ENSP00000429457 [100%] 
CTNNA1-014 - ENSP00000430626 [100%] 
CTNNA1-202 - ENSP00000486200 [100%] 

											 
																						CTNNA1-001 - ENSP00000304669 [100%] 
CTNNA1-006 - ENSP00000427821 [100%] 
CTNNA1-202 - ENSP00000486200 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CTNNA1-001
CTNNA1-006
CTNNA1-011
CTNNA1-014
CTNNA1-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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