CNTLN - Antibodies - The Human Protein Atlas

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CNTLN

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA007201

Antibody HPA036728

Antibody HPA036729

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA007201

HPA036728

HPA036729

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA007201

Antibody HPA036728

Antibody HPA036729

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA007201

Antibody HPA036728

Antibody HPA036729

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line AF22 shows localization to nucleoplasm, cytosol & centrosome.

Antibody dilution

1:8

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA007201

Antibody HPA036728

Antibody HPA036729

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 34 tissues
Protein: detected in 25 cell types

RNA: detected in 34 tissues
Protein: detected in 62 cell types

Retrieval

HIER pH6

Antibody dilution

1:60

1:750

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA007201

Antibody HPA036728

Antibody HPA036729

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

162.2

162.2, 44.6

162.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA007201

Antibody HPA036728

Antibody HPA036729

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

ANTIGEN INFORMATION

Antibody HPA007201

Antibody HPA036728

Antibody HPA036729

Antigen

Recombinant protein fragment

Length (aa)

148

101

Antigen sequence

ISELETEVTSLRRQVAEANALRNENEELINPMEKSHQSADRAKSEMATMK
VRSGRYDCKTTMTKVKFKAAKKNCSVGRHHTVLNHSIKVMSNVFENLSKD
GWEDVSESSSDSEAQTSQTLGTIIVETSQKISPTEDGKDQKESDPTED

LIEARKEVEVSQSKYNALSLQLSNKQTELIQKDMDITLVRKELQELQNLY
KQNSTHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESIS

SGMTEIRKIKRADPQQLRQEDSDAVWNELAYFKRENQELMIQKMNLEEEL
DELKVHISIDKAAIQELNRCVAERREEQLFRSGEDDEVKRSTPEKNGKEM
L

Matching transcripts

CNTLN-001 - ENSP00000370021 [100%]

CNTLN-001 - ENSP00000370021 [100%]
CNTLN-004 - ENSP00000370015 [100%]

CNTLN-001 - ENSP00000370021 [100%]

Other gene match

ANTIGEN VIEW

CNTLN-001	CNTLN-004

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.