CP - Antibodies - The Human Protein Atlas

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TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA001834

Antibody CAB008591

Provider

Atlas Antibodies
Sigma-Aldrich

AbFrontier

Product name

HPA001834

LF-MA0159

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Ammonium Sulfate Precipitation

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA001834

Antibody CAB008591

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA001834

Antibody CAB008591

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Immunohistochemical staining of human kidney shows distinct positivity in plasma.

Expression

RNA: detected in 32 tissues
Protein: detected in 57 cell types

RNA: detected in 32 tissues
Protein: detected in 39 cell types

Retrieval

HIER pH6

Antibody dilution

1:25

1:100

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001834

Antibody CAB008591

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

122.2, 97.7

122.2, 97.7, 26.1, 20.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA001834

Antibody CAB008591

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA001834

Antibody CAB008591

Antigen

Recombinant protein fragment

Native protein

Length (aa)

149

Antigen sequence

YFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGG
MKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHL
QEQNVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGI

Matching transcripts

CP-001 - ENSP00000264613 [100%]
CP-012 - ENSP00000420545 [100%]

Other gene match

ANTIGEN VIEW