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ZNF800

ANTIBODY INFORMATION
 
 

Antibody HPA023090

  

Antibody HPA052194

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA023090   HPA052194  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA023090

  

Antibody HPA052194

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023090

  

Antibody HPA052194

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nucleoli.  

Antibody dilution

 1:200   1:9  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023090

  

Antibody HPA052194

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong nucleolar positivity in Purkinje cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:2000    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA023090

  

Antibody HPA052194

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 75.2, 38.4, 27.5   75.2  

Antibody dilution

 1:250   1:90  

PROTEIN ARRAY
 
 

Antibody HPA023090

  

Antibody HPA052194

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:20000   1:700  

ANTIGEN INFORMATION
 
 

Antibody HPA023090

  

Antibody HPA052194

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 121   120  

Antigen sequence

 
PIETNQNAVFQYISRTDNPIEVTESSSTPEQTEVQIQETSTEQSKTVPVT
DTEVETVEPPPVEIVTDEVAPTSDEQPQESQADLETSDNSDFGHQLICCL
CRKEFNSRRGVRRHIRKVHKE
  
IRHITVVHKKSSRYLGKITASLEIRAIKKPIDFVLNKVAKRGPSRDEAKH
SDSKHDGTSNSPSKKYEVADVGIEVKVTKNFSLHRCNKCGKAFAKKTYLE
HHKKTHKANASNSPEGNKTK
 

Matching transcripts

 ZNF800-001 - ENSP00000265827 [99%]
ZNF800-002 - ENSP00000376989 [99%]
ZNF800-009 - ENSP00000376988 [99%]
ZNF800-201 - ENSP00000480871 [99%]
ZNF800-007 - ENSP00000403945 [89%]
  ZNF800-001 - ENSP00000265827 [100%]
ZNF800-002 - ENSP00000376989 [100%]
ZNF800-009 - ENSP00000376988 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF800-001
ZNF800-002
ZNF800-007
ZNF800-009
ZNF800-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.