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EPN3

ANTIBODY INFORMATION
 
 

Antibody HPA055546

  

Antibody HPA063224

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA055546   HPA063224  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA055546

  

Antibody HPA063224

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055546

  

Antibody HPA063224

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.  

Antibody dilution

   1:200  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055546

  

Antibody HPA063224

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 27 tissues
Protein: detected in 38 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:150    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA055546

  

Antibody HPA063224

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 68.2   68.2  

Antibody dilution

 1:270   1:950  

PROTEIN ARRAY
 
 

Antibody HPA055546

  

Antibody HPA063224

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2150   1:11450  

ANTIGEN INFORMATION
 
 

Antibody HPA055546

  

Antibody HPA063224

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   55  

Antigen sequence

 
SADPWDIPGFRPNTEASGSSWGPSADPWSPIPSGTVLSRSQPWDLTPMLS
SSEPWGRTPVLPAGPPTTDPWALNSPHHKLPST
  
LGPSASSLVNLDSLVKAPQVAKTRNPFLTGLSAPSPTNPFGAGEPGRPTL
NQMRT
 

Matching transcripts

 EPN3-001 - ENSP00000268933 [100%]
  EPN3-001 - ENSP00000268933 [100%]
 

Other gene match

    
ANTIGEN VIEW
EPN3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.