LTBP1 - Antibodies - The Human Protein Atlas

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LTBP1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA005707

Antibody CAB025428

Provider

Atlas Antibodies
Sigma-Aldrich

R&D Systems

Product name

HPA005707

MAB388

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA005707

Antibody CAB025428

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA005707

Antibody CAB025428

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & cytosol.

Antibody dilution

1:30

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA005707

Antibody CAB025428

Standard validation

Approved

Figure description

Immunohistochemical staining of human urinary bladder shows extracellular positivity in urothelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 34 cell types

Retrieval

HIER pH6

Antibody dilution

1:800

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA005707

Antibody CAB025428

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

186.8, 152.9, 148.4, 147.2, 147, 23.5

186.8, 152.9, 148.4, 147.2, 147, 29.3, 28.8, 23.5, 23.2, 10.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA005707

Antibody CAB025428

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

ANTIGEN INFORMATION

Antibody HPA005707

Antibody CAB025428

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

EKCPLPGTAKEEPVEALTFSREHGPGVAEPEVATAPPEKEIPSLDQEKTK
LEPGQPQLSPGISTIHLHPQFPVVIEKTSPPVPVEVAPEASTSS

Matching transcripts

LTBP1-001 - ENSP00000385359 [100%]
LTBP1-008 - ENSP00000384373 [100%]
LTBP1-007 - ENSP00000384091 [94%]
LTBP1-014 - ENSP00000386043 [94%]
LTBP1-201 - ENSP00000393057 [94%]
LTBP1-018 - ENSP00000417591 [92%]

Other gene match

ANTIGEN VIEW

LTBP1-001	LTBP1-007	LTBP1-008	LTBP1-014	LTBP1-018	LTBP1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.