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ANTIBODY INFORMATION
 
 

Antibody HPA018111

  

Antibody HPA056941

  

Antibody CAB010750

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA018111   HPA056941   E4013  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 4   12   3  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA018111

  

Antibody HPA056941

  

Antibody CAB010750

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018111

  

Antibody HPA056941

  

Antibody CAB010750

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.    

Antibody dilution

   1:64    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018111

  

Antibody HPA056941

  

Antibody CAB010750

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human skin shows distinct positivity in extracellular fibers.   Immunohistochemical staining of human testis shows distinct positivity in extracellular fibers.   Immunohistochemical staining of human skin shows distinct positivity in extracellular fibers.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 22 cell types		   RNA: detected in 36 tissues
Protein: detected in 1 cell types		   RNA: detected in 36 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:125   1:850  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018111

  

Antibody HPA056941

  

Antibody CAB010750

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 68.4, 66.1, 63.2, 62.6, 61.9, 61.6, 61.2, 61, 60.7, 59.9, 58.8, 56.5, 55.6, 26.2   68.4, 66.1, 63.2, 62.6, 61.9, 61, 60.7, 55.6   68.4, 66.1, 63.2, 62.6, 61.9, 61.6, 61.2, 61, 60.7, 59.9, 58.8, 56.5, 55.6, 53.6, 48.9, 26.2, 19.3, 15.7, 15.2, 14.7, 13.8  

Antibody dilution

 1:250   1:210   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018111

  

Antibody HPA056941

  

Antibody CAB010750

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA018111

  

Antibody HPA056941

  

Antibody CAB010750

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 135   72    

Antigen sequence

 
VKPGKVPGVGLPGVYPGGVLPGARFPGVGVLPGVPTGAGVKPKAPGVGGA
FAGIPGVGPFGGPQPGVPLGYPIKAPKLPGGYGLPYTTGKLPYGYGPGGV
AGAAGKAGYPTGTGVGPQAAAAAAAKAAAKFGAGA
  
VGGLGVPGVGGLGGIPPAAAAKAAKYGAAGLGGVLGGAGQFPLGGVAARP
GFGLSPIFPGGACLGKACGRKR
  

											 
																				

																		

										
											
Matching transcripts

												
																					ELN-001 - ENSP00000313565 [100%] 
ELN-002 - ENSP00000252034 [100%] 
ELN-003 - ENSP00000389857 [100%] 
ELN-005 - ENSP00000392575 [100%] 
ELN-006 - ENSP00000369936 [100%] 
ELN-007 - ENSP00000369949 [100%] 
ELN-009 - ENSP00000403162 [100%] 
ELN-010 - ENSP00000349540 [100%] 
ELN-011 - ENSP00000391129 [100%] 
ELN-013 - ENSP00000369950 [100%] 
ELN-201 - ENSP00000351807 [100%] 
ELN-015 - ENSP00000406949 [93%] 
ELN-008 - ENSP00000369958 [90%] 
ELN-004 - ENSP00000315607 [82%] 

											 
																						ELN-001 - ENSP00000313565 [100%] 
ELN-002 - ENSP00000252034 [100%] 
ELN-004 - ENSP00000315607 [100%] 
ELN-005 - ENSP00000392575 [100%] 
ELN-006 - ENSP00000369936 [100%] 
ELN-009 - ENSP00000403162 [100%] 
ELN-013 - ENSP00000369950 [100%] 
ELN-201 - ENSP00000351807 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

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ELN-001
ELN-002
ELN-003
ELN-004
ELN-005
ELN-006
ELN-007
ELN-008
ELN-009
ELN-010
ELN-011
ELN-013
ELN-015
ELN-201
								

							

						

							
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RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA018111

												
										 
																				
											
											
											
Antibody HPA056941

												
										 
																				
											
											
											
Antibody CAB010750

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Elastogenesis at the onset of human cardiac valve development

	Votteler M et al

	Development 2013;140(11):2345-53

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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