We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA056010 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA055409 across 74 cell types.
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human epididymis shows granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human epididymis shows granular cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 20 cell types
RNA: detected in 37 tissues Protein: detected in 24 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:60
1:10
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055409
Antibody HPA056010
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424662)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
63.1
63.1
Antibody dilution
1:125
1:100
PROTEIN ARRAY
Antibody HPA055409
Antibody HPA056010
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
