LIMA1 - Antibodies - The Human Protein Atlas

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LIMA1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA023871

Antibody HPA052645

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA023871

HPA052645

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA023871

Antibody HPA052645

Immunocytochemistry

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA023871

Antibody HPA052645

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA052645.

Validated

Antibody staining overlaps with antibody HPA023871.

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & actin filaments.

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, actin filaments & focal adhesion sites.

Antibody dilution

1:10

1:75

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA023871

Antibody HPA052645

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA052645 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA023871 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA052645 across 71 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA023871 across 71 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 57 cell types

RNA: detected in 37 tissues
Protein: detected in 46 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:1000

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA023871

Antibody HPA052645

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

85.2, 67.1, 67

85.2, 67.1, 67, 51.2, 51.1

Antibody dilution

1:250

1:380

PROTEIN ARRAY

Antibody HPA023871

Antibody HPA052645

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

ANTIGEN INFORMATION

Antibody HPA023871

Antibody HPA052645

Antigen

Recombinant protein fragment

Length (aa)

123

Antigen sequence

ISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAK
YQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGE
KISANENSLAVRSTPAEDDSRDS

RPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASM
EAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGI

Matching transcripts

LIMA1-003 - ENSP00000340184 [100%]
LIMA1-004 - ENSP00000450087 [100%]
LIMA1-006 - ENSP00000450266 [100%]
LIMA1-001 - ENSP00000378400 [98%]
LIMA1-002 - ENSP00000448779 [98%]

LIMA1-001 - ENSP00000378400 [100%]
LIMA1-002 - ENSP00000448779 [100%]
LIMA1-003 - ENSP00000340184 [100%]
LIMA1-004 - ENSP00000450087 [100%]
LIMA1-006 - ENSP00000450266 [100%]
LIMA1-007 - ENSP00000448706 [100%]
LIMA1-017 - ENSP00000448463 [100%]

Other gene match

ANTIGEN VIEW

LIMA1-001	LIMA1-002	LIMA1-003	LIMA1-004	LIMA1-006	LIMA1-007	LIMA1-017

RELEVANT PUBLICATIONS

Antibody HPA023871

Antibody HPA052645

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.