We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
Antibody dilution
1:20
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038395
Antibody HPA038396
Antibody CAB032519
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 78 cell types
RNA: detected in 37 tissues Protein: detected in 49 cell types
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:10
1:100
1:110
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038395
Antibody HPA038396
Antibody CAB032519
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
92.2, 92.1
92.2, 92.1
92.2, 92.1, 18.3
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA038395
Antibody HPA038396
Antibody CAB032519
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
