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PTPRN

ANTIBODY INFORMATION
 
 

Antibody HPA007152

  

Antibody HPA007179

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA007152   HPA007179  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   3  

References

   1  

VALIDATION SUMMARY
 
 

Antibody HPA007152

  

Antibody HPA007179

  
Immunocytochemistry

Approved    
Immunohistochemistry

 

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007152

  

Antibody HPA007179

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.    

Antibody dilution

 1:8    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007152

  

Antibody HPA007179

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of adrenal gland shows strong cytoplasmic positivity in medullary cells.  

Expression

   RNA: detected in 11 tissues
Protein: detected in 8 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:250  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007152

  

Antibody HPA007179

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 105.8, 102.8, 96.3   105.8, 102.8, 96.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA007152

  

Antibody HPA007179

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA007152

  

Antibody HPA007179

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 113   113  

Antigen sequence

 
HSYGDLPGPSPAQLFQDSGLLYLAQELPAPSRARVPRLPEQGSSSRAEDS
PEGYEKEGLGDRGEKPASPAVQPDAALQRLAAVLAGYGVELRQLTPEQLS
TLLTLLQLLPKGA
  
HSYGDLPGPSPAQLFQDSGLLYLAQELPAPSRARVPRLPEQGSSSRAEDS
PEGYEKEGLGDRGEKPASPAVQPDAALQRLAAVLAGYGVELRQLTPEQLS
TLLTLLQLLPKGA
 

Matching transcripts

 PTPRN-001 - ENSP00000295718 [100%]
PTPRN-003 - ENSP00000386638 [100%]
PTPRN-021 - ENSP00000392598 [100%]
  PTPRN-001 - ENSP00000295718 [100%]
PTPRN-003 - ENSP00000386638 [100%]
PTPRN-021 - ENSP00000392598 [100%]
 

Other gene match

    
ANTIGEN VIEW
PTPRN-001
PTPRN-003
PTPRN-021

RELEVANT PUBLICATIONS
 
 

Antibody HPA007152

  

Antibody HPA007179

  

1.

   Novel pancreatic beta cell-specific proteins: antibody-based proteomics for identification of new biomarker candidates
Lindskog C et al
J Proteomics 2012;75(9):2611-20
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.