CYFIP2 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

CYFIP2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA068106

Antibody HPA071459

Antibody CAB055519

Provider

Atlas Antibodies
Sigma-Aldrich

Upstate

Product name

HPA068106

HPA071459

07-531

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

CYFIP1 - ENSG00000273749 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA068106

Antibody HPA071459

Antibody CAB055519

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA068106

Antibody HPA071459

Antibody CAB055519

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & cytosol.

Antibody dilution

1:21

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA068106

Antibody HPA071459

Antibody CAB055519

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic and nucleolar positivity in neuronal cells.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 64 cell types

RNA: detected in 37 tissues
Protein: detected in 68 cell types

Retrieval

HIER pH6

Antibody dilution

1:25

1:700

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA068106

Antibody HPA071459

Antibody CAB055519

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

148.4, 145.7, 145.2, 142.6, 34.4, 25.9

148.4

148.4, 145.7, 142.6, 129.7, 122.5, 34.4, 27.4, 25.9, 21.9

Antibody dilution

1:180

1:80

1:500

PROTEIN ARRAY

Antibody HPA068106

Antibody HPA071459

Antibody CAB055519

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:550

ANTIGEN INFORMATION

Antibody HPA068106

Antibody HPA071459

Antibody CAB055519

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

TLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQN
LSMFLANHN

STQACQWSPRALFHPTGGTQGRRGCRSLLY

Matching transcripts

CYFIP2-001 - ENSP00000430904 [100%]
CYFIP2-002 - ENSP00000484819 [100%]
CYFIP2-003 - ENSP00000479719 [100%]
CYFIP2-008 - ENSP00000479968 [100%]
CYFIP2-013 - ENSP00000480605 [100%]
CYFIP2-015 - ENSP00000478058 [100%]

CYFIP2-003 - ENSP00000479719 [100%]

Other gene match

CYFIP1 - ENSG00000273749 [100%]

ANTIGEN VIEW

CYFIP2-001	CYFIP2-002	CYFIP2-003	CYFIP2-008	CYFIP2-013	CYFIP2-015

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.