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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:25
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001835
Antibody HPA003948
Antibody CAB072872
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human epididymis shows strong positivity in plasma.
Immunohistochemical staining of human epididymis shows strong positivity in plasma.
Expression
RNA: detected in 32 tissues Protein: detected in 16 cell types
RNA: detected in 32 tissues Protein: detected in 2 cell types
RNA: detected in 32 tissues Protein: detected in 1 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:600
1:2500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001835
Antibody HPA003948
Antibody CAB072872
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
103.9, 103.4, 99.9, 80
103.4, 99.9, 80
103.9, 103.4, 99.9, 80
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA001835
Antibody HPA003948
Antibody CAB072872
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.