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U2AF2

ANTIBODY INFORMATION
 
 

Antibody HPA041943

  

Antibody HPA043562

  

Antibody CAB010910

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA041943   HPA043562   U4758  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 12   12   3  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA041943

  

Antibody HPA043562

  

Antibody CAB010910

  
Immunocytochemistry

Supported     Supported  
Immunohistochemistry

  Supported   Supported  
Western blot

Supported  

Validated
   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041943

  

Antibody HPA043562

  

Antibody CAB010910

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles.     Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear speckles.  

Antibody dilution

 1:69     1:175  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041943

  

Antibody HPA043562

  

Antibody CAB010910

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong nuclear positivity in cells of tubules.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:70   1:700  

Literature conformity

   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041943

  

Antibody HPA043562

  

Antibody CAB010910

  

Formal validation: Genetic

  
Validated

Downregulation visible in one of two siRNA lanes		    

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

   53.5, 53.1, 33.9    

Loading control

   Total protein image    

Antibody dilution

   1:58    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 53.5, 53.1   53.5, 53.1, 33.9   53.5, 53.1, 33.9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA041943

  

Antibody HPA043562

  

Antibody CAB010910

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA041943

  

Antibody HPA043562

  

Antibody CAB010910

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 100   92    

Antigen sequence

 
LTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAG
QIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEA
  
PDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAF
CEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVS
   

Matching transcripts

 U2AF2-001 - ENSP00000388475 [100%]
U2AF2-002 - ENSP00000307863 [100%]
  U2AF2-002 - ENSP00000307863 [100%]
U2AF2-001 - ENSP00000388475 [98%]
U2AF2-008 - ENSP00000466935 [98%]
   

Other gene match

      
ANTIGEN VIEW
U2AF2-001
U2AF2-002
U2AF2-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.