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MTMR1

ANTIBODY INFORMATION
 
 

Antibody HPA002977

  

Antibody HPA018502

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002977   HPA018502  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA002977

  

Antibody HPA018502

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

  Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002977

  

Antibody HPA018502

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & vesicles.    

Antibody dilution

 1:4    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002977

  

Antibody HPA018502

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:30  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002977

  

Antibody HPA018502

  

Standard validation

   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

   75.7, 74.7, 39.8, 19.5  

Antibody dilution

   1:250  

PROTEIN ARRAY
 
 

Antibody HPA002977

  

Antibody HPA018502

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA002977

  

Antibody HPA018502

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 141   120  

Antigen sequence

 
HAFPLSNGQALFAFSYKEKFPINGWKVYDPVSEYKRQGLPNESWKISKIN
SNYEFCDTYPAIIVVPTSVKDDDLSKVAAFRAKGRVPVLSWIHPESQATI
TRCSQPLVGPNDKRCKEDEKYLQTIMDANAQSHKLIIFDAR
  
GRRPPRAAGGATAGSRQPSVETLDSPTGSHVEWCKQLIAATISSQISGSV
TSENVSRDYKALRDGNKLAQMEEAPLFPGESIKAIVKDVMYICPFMGAVS
GTLTVTDFKLYFKNVERDPH
 

Matching transcripts

 MTMR1-002 - ENSP00000414178 [100%]
MTMR1-013 - ENSP00000359417 [100%]
MTMR1-201 - ENSP00000445281 [100%]
  MTMR1-013 - ENSP00000359417 [100%]
MTMR1-201 - ENSP00000445281 [100%]
MTMR1-002 - ENSP00000414178 [94%]
MTMR1-008 - ENSP00000436957 [88%]
 

Other gene match

    
ANTIGEN VIEW
MTMR1-002
MTMR1-008
MTMR1-013
MTMR1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.