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TNPO3

ANTIBODY INFORMATION
 
 

Antibody HPA039555

  

Antibody HPA041537

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039555   HPA041537  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA039555

  

Antibody HPA041537

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039555

  

Antibody HPA041537

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA041537.		  
Validated

Antibody staining overlaps with antibody HPA039555.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.   Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.  

Antibody dilution

 1:9   1:64  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039555

  

Antibody HPA041537

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine pancreas.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:15    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA039555

  

Antibody HPA041537

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 108, 104.2, 97.4, 96.7   108, 104.2, 97.4, 96.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA039555

  

Antibody HPA041537

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA039555

  

Antibody HPA041537

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 119   110  

Antigen sequence

 
VRCVGKGSAALLQPLVTQMVNVYHVHQHSCFLYLGSILVDEYGMEEGCRQ
GLLDMLQALCIPTFQLLEQQNGLQNHPDTVDDLFRLATRFIQRSPVTLLR
SQVVIPILQWAIASTTLDH
  
RSLDSFLLSPEAAVGLLKGTALVLARLPLDKITECLSELCSVQVMALKKL
LSQEPSNGISSDPTVFLDRLAVIFRHTNPIVENGQTHPCQKVIQEIWPVL
SETLNKHRAD
 

Matching transcripts

 TNPO3-001 - ENSP00000265388 [100%]
TNPO3-002 - ENSP00000420089 [100%]
TNPO3-003 - ENSP00000418646 [100%]
TNPO3-005 - ENSP00000418267 [100%]
TNPO3-201 - ENSP00000487231 [100%]
  TNPO3-001 - ENSP00000265388 [100%]
TNPO3-002 - ENSP00000420089 [100%]
TNPO3-005 - ENSP00000418267 [100%]
TNPO3-201 - ENSP00000487231 [100%]
TNPO3-003 - ENSP00000418646 [84%]
 

Other gene match

    
ANTIGEN VIEW
TNPO3-001
TNPO3-002
TNPO3-003
TNPO3-005
TNPO3-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.