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Antibody staining overlaps with antibody HPA053330.
Validated
Antibody staining overlaps with antibody HPA049157.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Antibody dilution
1:79
1:78
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA049157
Antibody HPA053330
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules and strong nuclear staining in cells in glomeruli.
Expression
RNA: detected in 37 tissues Protein: detected in 80 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049157
Antibody HPA053330
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
28.1, 25.7, 25.6, 20.2, 15.7, 8.7
28.1, 15.7
Antibody dilution
1:390
1:390
PROTEIN ARRAY
Antibody HPA049157
Antibody HPA053330
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
