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ABCA7

ANTIBODY INFORMATION
 
 

Antibody HPA041564

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041564  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA041564

  
Immunocytochemistry

Supported  
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041564

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & cell junctions.  

Antibody dilution

 1:200  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041564

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:1500  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041564

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 234.3, 218.5  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA041564

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA041564

  

Antigen

 Recombinant protein fragment  

Length (aa)

 86  

Antigen sequence

 
VNRTFEELTLLRDVREVWEMLGPRIFTFMNDSSNVAMLQRLLQMQDEGRR
QPRPGGRDHMEALRSFLDPGSGGYSWQDAHADVGHL
 

Matching transcripts

 ABCA7-001 - ENSP00000414062 [100%]
ABCA7-002 - ENSP00000465322 [100%]
ABCA7-201 - ENSP00000263094 [100%]
 

Other gene match

  
ANTIGEN VIEW
ABCA7-001
ABCA7-002
ABCA7-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.