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MCM10

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GENERAL INFORMATION

Gene name

 MCM10

Gene description

 Minichromosome maintenance 10 replication initiation factor

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 4
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Localized to the nucleus & nucleoli.

RNA cell category

 Mixed

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleus (supported), Nucleoli (supported)

Single-cell variation

 Single-cell variation in protein expression observed.

Cell cycle dependency

 Cell cycle dependent expression determined by custom assay

DATA RELIABILITY

Reliability score

 Supported based on 1 antibody.
HPA071952
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ASSAYS
RNA expression
Human cells
Custom data

RNA EXPRESSION OVERVIEW
RNA cell line category: Mixed
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Localized to the nucleus & nucleoli.

Main location

 Nucleus (supported), Nucleoli (supported)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA071952 HaCaT Nucleoplasm
HPA071952 RH-30 Nucleoplasm
HPA071952 U-2 OS Nucleus Intensity Intensity
Nucleoli Intensity Intensity

CUSTOM DATA

 

 

 
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Thumbnail

Antibody

Cell line

Assay

Description
HPA071952 U-2 OS Image Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. MCM10 is mostly expressed during G1.

CELL CYCLE

Summary

 Expression has cell cycle dependency according to custom assay

Intensity variation

 Nucleoli, Nucleus

GENE INFORMATION

Gene name

 MCM10 (HGNC Symbol)

Synonyms

 CNA43, DNA43, PRO2249

Description

 Minichromosome maintenance 10 replication initiation factor (HGNC Symbol)

Entrez gene summary

 The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Chromosome

 10

Cytoband

 p13

Chromosome location (bp)

 13161554 - 13211104

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000065328 (version 83.38)

Entrez gene

 55388

UniProt

 Q7L590 (UniProt - Evidence at protein level)

neXtProt

 NX_Q7L590

Antibodypedia

 MCM10 antibodies

PROTEIN BROWSER
MCM10-001
MCM10-002
MCM10-003
MCM10-004

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
MCM10-001
ENSP00000367986
ENST00000378714
Q7L590 [Direct mapping]
Protein MCM10 homolog
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0003688 [DNA replication origin binding]
GO:0003690 [double-stranded DNA binding]
GO:0003697 [single-stranded DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0006260 [DNA replication]
GO:0006270 [DNA replication initiation]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0031298 [replication fork protection complex]
GO:0042802 [identical protein binding]
GO:0046872 [metal ion binding]
Show all
 874 aa
98.1 kDa 		No 0
MCM10-002
ENSP00000367966
ENST00000378694
Q5T670 [Direct mapping]
MCM10 minichromosome maintenance deficient 10 (S. cerevisiae), isoform CRA_b; Protein MCM10 homolog
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003688 [DNA replication origin binding]
GO:0003690 [double-stranded DNA binding]
GO:0003697 [single-stranded DNA binding]
GO:0005634 [nucleus]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0006260 [DNA replication]
GO:0006270 [DNA replication initiation]
GO:0031298 [replication fork protection complex]
Show all
 855 aa
96.1 kDa 		No 0
MCM10-003
ENSP00000418268
ENST00000484800
Q7L590 [Direct mapping]
Protein MCM10 homolog
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0003688 [DNA replication origin binding]
GO:0003690 [double-stranded DNA binding]
GO:0003697 [single-stranded DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0006260 [DNA replication]
GO:0006270 [DNA replication initiation]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0008283 [cell proliferation]
GO:0031298 [replication fork protection complex]
GO:0042802 [identical protein binding]
GO:0046872 [metal ion binding]
Show all
 875 aa
98.2 kDa 		No 0
MCM10-004
ENSP00000417094
ENST00000479669
C9J600 [Direct mapping]
Protein MCM10 homolog
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003688 [DNA replication origin binding]
GO:0003690 [double-stranded DNA binding]
GO:0003697 [single-stranded DNA binding]
GO:0006270 [DNA replication initiation]
GO:0031298 [replication fork protection complex]
Show all
 69 aa
7.9 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.