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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity neuronal cells and strong staining in neuropil.
Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neuronal cells and strong staining in neuropil.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells, cells in molecular layer and cells in granular layer.
Expression
RNA: detected in 15 tissues Protein: detected in 45 cell types
RNA: detected in 15 tissues Protein: detected in 31 cell types
RNA: detected in 15 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:900
1:50
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029632
Antibody HPA029633
Antibody CAB009745
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil