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DDX3Y

ANTIBODY INFORMATION
 
 

Antibody HPA001648

  

Antibody HPA005631

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001648   HPA005631  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 DDX3X - ENSG00000215301 [100%]
   DDX3X - ENSG00000215301 [100%]
  

Released in version

 1   3  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA001648

  

Antibody HPA005631

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001648

  

Antibody HPA005631

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

 1:15   1:29  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001648

  

Antibody HPA005631

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in reaction center cells in lymphoid tissue.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 28 tissues
Protein: detected in 45 cell types		   RNA: detected in 28 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  
 

Standard validation

 Approved    

   

Figure description

 Cytoplasmic staining in subsets of neurons, most abundant in cortical regions.    

WESTERN BLOT
 
 

Antibody HPA001648

  

Antibody HPA005631

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 81.5, 73.2, 71.4, 70.8, 27.7, 25, 17.8   81.5, 73.2, 71.4, 70.8, 14.7  

Antibody dilution

 1:500   1:250  

PROTEIN ARRAY
 
 

Antibody HPA001648

  

Antibody HPA005631

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA001648

  

Antibody HPA005631

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 148   124  

Antigen sequence

 
LNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYS
SFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFE
RGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPV
  
LPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEV
PSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSR
ASSSRSGGGGHGSSRGFGGGGYGG
 

Matching transcripts

 DDX3Y-001 - ENSP00000353284 [85%]
DDX3Y-003 - ENSP00000336725 [85%]
DDX3Y-004 - ENSP00000398953 [85%]
DDX3Y-005 - ENSP00000400377 [85%]
  DDX3Y-001 - ENSP00000353284 [91%]
DDX3Y-003 - ENSP00000336725 [91%]
 

Other gene match

 DDX3X - ENSG00000215301 [100%]
   DDX3X - ENSG00000215301 [100%]
  
ANTIGEN VIEW
DDX3Y-001
DDX3Y-003
DDX3Y-004
DDX3Y-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.