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Antibody staining overlaps with antibody HPA053724.
Validated
Antibody staining overlaps with antibody HPA027339.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.
Antibody dilution
1:50
1:133
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA027339
Antibody HPA053724
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.
Expression
RNA: detected in 35 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027339
Antibody HPA053724
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
94.1, 92.9
94.1, 92.9
Antibody dilution
1:250
1:660
PROTEIN ARRAY
Antibody HPA027339
Antibody HPA053724
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
