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Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
Antibody dilution
1:51
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA012285
Antibody HPA066549
Standard validation
Supported
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Expression
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA012285
Antibody HPA066549
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417238)
Target mass (kDa)
47.8
47.8, 44.9, 33
Antibody dilution
1:250
1:170
PROTEIN ARRAY
Antibody HPA012285
Antibody HPA066549
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
