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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA049864
Standard validation
Supported
Figure description
Immunohistochemical staining of pituitary gland shows strong cytoplasmic positivity in subsets of cells.
Expression
RNA: detected in 11 tissues Protein: detected in 2 cell types
Retrieval
HIER pH6
Antibody dilution
1:1100
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049864
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
13.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA049864
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
