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PHRF1

ANTIBODY INFORMATION
 
 

Antibody HPA019867

  

Antibody HPA062933

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019867   HPA062933  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA019867

  

Antibody HPA062933

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019867

  

Antibody HPA062933

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human cerebellum shows moderate nuclear and cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 16 tissues
Protein: detected in 15 cell types		   RNA: detected in 16 tissues
Protein: detected in 29 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:500  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019867

  

Antibody HPA062933

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 178.7, 178.6, 178.4, 178.2   178.7, 178.6, 178.4, 178.2  

Antibody dilution

 1:250   1:190  

PROTEIN ARRAY
 
 

Antibody HPA019867

  

Antibody HPA062933

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2250  

ANTIGEN INFORMATION
 
 

Antibody HPA019867

  

Antibody HPA062933

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 138   82  

Antigen sequence

 
SCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLS
PLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDL
LGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQR
  
ASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRA
PLHRPQKPREGAWDMEDVAPTGVRQAFSELPF
 

Matching transcripts

 PHRF1-001 - ENSP00000264555 [100%]
PHRF1-003 - ENSP00000431870 [100%]
PHRF1-007 - ENSP00000388589 [99%]
PHRF1-008 - ENSP00000410626 [99%]
  PHRF1-001 - ENSP00000264555 [99%]
PHRF1-003 - ENSP00000431870 [99%]
PHRF1-007 - ENSP00000388589 [99%]
PHRF1-008 - ENSP00000410626 [99%]
 

Other gene match

    
ANTIGEN VIEW
PHRF1-001
PHRF1-003
PHRF1-007
PHRF1-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.