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NUCB2

ANTIBODY INFORMATION
 
 

Antibody HPA008395

  

Antibody CAB015931

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Apotech Corp.
  

Product name

 HPA008395   ALX-804-854-C100  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 4   5  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA008395

  

Antibody CAB015931

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008395

  

Antibody CAB015931

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.  

Antibody dilution

 1:83   1:20  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008395

  

Antibody CAB015931

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		   RNA: detected in 37 tissues
Protein: detected in 58 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:2500   1:80  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008395

  

Antibody CAB015931

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.2   50.2, 46.6, 23.7, 23.6, 23.2, 10.5, 8.5, 8, 4.6, 4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008395

  

Antibody CAB015931

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA008395

  

Antibody CAB015931

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 118    

Antigen sequence

 
MSEVDTNKDRLVTLEEFLKATEKKEFLEPDSWETLDQQQFFTEEELKEYE
NIIALQENELKKKADELQKQKEELQRQHDQLEAQKLEYHQVIQQMEQKKL
QQGIPPSGPAGELKFEPR
  

											 
																				

																		

										
											
Matching transcripts

												
																					NUCB2-004 - ENSP00000320168 [99%] 
NUCB2-005 - ENSP00000436455 [98%] 
NUCB2-202 - ENSP00000481959 [98%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NUCB2-004
NUCB2-005
NUCB2-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008395

												
										 
																				
											
											
											
Antibody CAB015931

												
										 
																		

																	

										
											
1.

												
																						Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

	Stadler C et al

	J Proteomics 2012;75(7):2236-51

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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