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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
Antibody dilution
1:200
1:196
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027257
Antibody HPA063869
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows moderate positivity in subsets of cells in sminiferus ducts.
Expression
RNA: detected in 36 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027257
Antibody HPA063869
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
63.6, 60.3
63.6, 60.3
Antibody dilution
1:250
1:660
PROTEIN ARRAY
Antibody HPA027257
Antibody HPA063869
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
