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TCOF1

ANTIBODY INFORMATION
 
 

Antibody HPA038237

  

Antibody HPA038238

  

Antibody CAB033199

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA038237   HPA038238   sc-49529  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 7   7   6  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA038237

  

Antibody HPA038238

  

Antibody CAB033199

  
Immunocytochemistry


Validated

Validated
  

Validated
    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038237

  

Antibody HPA038238

  

Antibody CAB033199

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		      

 

Relative fluorescence intensity (RFI)

      

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:76      
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA038238.		  
Validated

Antibody staining overlaps with antibody HPA038237.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.    

Antibody dilution

 1:76   1:26    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038237

  

Antibody HPA038238

  

Antibody CAB033199

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human rectum shows moderate nucleolar and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:25   1:30  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038237

  

Antibody HPA038238

  

Antibody CAB033199

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 152.3, 152.1, 148.4, 144.4, 144.3, 144.1, 96.8   152.1, 144.4, 144.3   152.3, 152.1, 148.4, 144.4, 144.3, 144.1, 96.8, 21.1, 6.8  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038237

  

Antibody HPA038238

  

Antibody CAB033199

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA038237

  

Antibody HPA038238

  

Antibody CAB033199

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 84   85    

Antigen sequence

 
KSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSAD
SEEAPAAMTAAQAKPALKIPQTKACPKKTNTTAS
  
ESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVART
QPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPK
  

											 
																				

																		

										
											
Matching transcripts

												
																					TCOF1-001 - ENSP00000325223 [100%] 
TCOF1-002 - ENSP00000406888 [100%] 
TCOF1-003 - ENSP00000409944 [100%] 
TCOF1-004 - ENSP00000377811 [100%] 
TCOF1-008 - ENSP00000427484 [100%] 
TCOF1-014 - ENSP00000421655 [100%] 
TCOF1-015 - ENSP00000390717 [100%] 
TCOF1-201 - ENSP00000367028 [100%] 

											 
																						TCOF1-001 - ENSP00000325223 [100%] 
TCOF1-003 - ENSP00000409944 [100%] 
TCOF1-014 - ENSP00000421655 [100%] 
TCOF1-201 - ENSP00000367028 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TCOF1-001
TCOF1-002
TCOF1-003
TCOF1-004
TCOF1-008
TCOF1-014
TCOF1-015
TCOF1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA038237

												
										 
																				
											
											
											
Antibody HPA038238

												
										 
																				
											
											
											
Antibody CAB033199

												
										 
																		

																	

										
											
1.

												
																						The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage

	Larsen DH et al

	Nat Cell Biol 2014;16(8):792-803

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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