HLTF - Antibodies - The Human Protein Atlas

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HLTF

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA015284

Antibody HPA049001

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA015284

HPA049001

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA015284

Antibody HPA049001

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA015284

Antibody HPA049001

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA049001.

Validated

Antibody staining overlaps with antibody HPA015284.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line A-431 shows localization to nucleus.

Antibody dilution

1:33

1:34

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA015284

Antibody HPA049001

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human testis shows nuclear positivity of cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 14 cell types

Retrieval

HIER pH6

Antibody dilution

1:40

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA015284

Antibody HPA049001

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

113.9

Antibody dilution

1:250

1:170

PROTEIN ARRAY

Antibody HPA015284

Antibody HPA049001

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:1350

ANTIGEN INFORMATION

Antibody HPA015284

Antibody HPA049001

Antigen

Recombinant protein fragment

Length (aa)

137

134

Antigen sequence

LKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTE
FDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPF
WEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGK

MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLT
SDEEVDSVLFGSLRGHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKV
NNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVV

Matching transcripts

HLTF-001 - ENSP00000420429 [100%]
HLTF-002 - ENSP00000420745 [100%]
HLTF-003 - ENSP00000308944 [100%]
HLTF-004 - ENSP00000376644 [100%]

Other gene match

ANTIGEN VIEW

HLTF-001	HLTF-002	HLTF-003	HLTF-004

RELEVANT PUBLICATIONS

Antibody HPA015284

Antibody HPA049001

Role of helicase-like transcription factor (hltf) in the G2/m transition and apoptosis in brain
Helmer RA et al
PLoS One 2013;8(6):e66799

Helicase-like transcription factor: a new marker of well-differentiated thyroid cancers
Arcolia V et al
BMC Cancer 2014;14:492
Application: IHC, WB

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.