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FAM50A

ANTIBODY INFORMATION
 
 

Antibody HPA003585

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003585  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 2  

References

 1  

VALIDATION SUMMARY
 
 

Antibody HPA003585

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Supported  
Western blot


Validated
  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003585

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:122  
 

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:118  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003585

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:500  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003585

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		  

 

Figure description

 Lane 1: Marker [kDa] 250, 150, 100, 75, 50, 37, 25, 20, 15, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

 40.2, 30.7  

Loading control

 Total protein image  

Antibody dilution

 1:250  
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 40.2, 30.7  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA003585

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA003585

  

Antigen

 Recombinant protein fragment  

Length (aa)

 106  

Antigen sequence

 
VTLNDMKAKQEALVKEREKQLAKKEQSKELQMKLEKLREKERKKEAKRKI
SSLSFTLEEEEEGGEEEEEAAMYEEEMEREEITTKKRKLGKNPDVDTSFL
PDRDRE
 

Matching transcripts

 FAM50A-001 - ENSP00000377225 [100%]
FAM50A-002 - ENSP00000158526 [100%]
 

Other gene match

  
ANTIGEN VIEW
FAM50A-001
FAM50A-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA003585

  

1.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.