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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane, cytoplasm & nucleus but excluded from the nucleoli and GFP expression in nucleus but excluded from the nucleoli.
Antibody dilution
1:39
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:40
1:62
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA005499
Antibody CAB025404
Standard validation
Supported
Figure description
Immunohistochemical staining of human small intestine shows distinct nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA005499
Antibody CAB025404
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Target mass (kDa)
143.2, 140.9, 31.4
143.2, 140.9, 31.4, 30.5
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA005499
Antibody CAB025404
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
