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FCGR2B

ANTIBODY INFORMATION
 
 

Antibody HPA014730

  

Antibody CAB007796

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Epitomics
  

Product name

 HPA014730   1723-1  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 FCGR2A - ENSG00000143226 [90%]
    

Released in version

 12   3  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA014730

  

Antibody CAB007796

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014730

  

Antibody CAB007796

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows strong positivity in endothelial cells.   Immunohistochemical staining of human placenta shows strong positivity in endothelial cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 16 cell types		   RNA: detected in 36 tissues
Protein: detected in 23 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:60   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA014730

  

Antibody CAB007796

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 35, 34.9, 34, 33.4, 31.9   34, 33.4, 31.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA014730

  

Antibody CAB007796

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA014730

  

Antibody CAB007796

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 40    

Antigen sequence

 
PKAVLKLEPQWINVLQEDSVTLTCRGTHSPESDSIQWFHN
   

Matching transcripts

 FCGR2B-001 - ENSP00000356938 [100%]
FCGR2B-002 - ENSP00000351497 [100%]
FCGR2B-005 - ENSP00000236937 [100%]
FCGR2B-201 - ENSP00000404329 [100%]
   

Other gene match

 FCGR2A - ENSG00000143226 [90%]
    
ANTIGEN VIEW
FCGR2B-001
FCGR2B-002
FCGR2B-005
FCGR2B-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.