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DERL2

ANTIBODY INFORMATION
 
 

Antibody HPA048957

  

Antibody HPA056551

  

Antibody CAB037287

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA048957   HPA056551   D1194  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 11   11   7  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA048957

  

Antibody HPA056551

  

Antibody CAB037287

  
Immunocytochemistry

    Supported  
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048957

  

Antibody HPA056551

  

Antibody CAB037287

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.  

Antibody dilution

     1:50  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048957

  

Antibody HPA056551

  

Antibody CAB037287

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA056551 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA048957 across 46 cell lines.		    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA056551 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA048957 across 74 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of germinal and non-germinal center cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of germinal and non-germinal center cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of germinal and non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 47 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:30   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048957

  

Antibody HPA056551

  

Antibody CAB037287

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 27.6, 18   27.6, 18   27.6, 23.4, 18, 17.2, 8.8  

Antibody dilution

 1:380   1:280   1:500  

PROTEIN ARRAY
 
 

Antibody HPA048957

  

Antibody HPA056551

  

Antibody CAB037287

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:30000   1:2250    

ANTIGEN INFORMATION
 
 

Antibody HPA048957

  

Antibody HPA056551

  

Antibody CAB037287

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 23   23    

Antigen sequence

 
NYNPLPEERPGGFAWGEGQRLGG
  
GIRILKTPSILKAIFDTPDEDPN
  

											 
																				

																		

										
											
Matching transcripts

												
																					DERL2-001 - ENSP00000158771 [100%] 
DERL2-012 - ENSP00000459083 [100%] 

											 
																						DERL2-001 - ENSP00000158771 [100%] 
DERL2-012 - ENSP00000459083 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DERL2-001
DERL2-012
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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