TP63 - Antibodies - The Human Protein Atlas

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TP63

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA006288

Antibody HPA007010

Antibody CAB000083

Provider

Atlas Antibodies
Sigma-Aldrich

DakoCytomation

Product name

HPA006288

HPA007010

M7247

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA006288

Antibody HPA007010

Antibody CAB000083

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA006288

Antibody HPA007010

Antibody CAB000083

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:137

1:10

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA006288

Antibody HPA007010

Antibody CAB000083

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 35 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human urinary bladder shows strong nucelar positivity in urothelial cells.

Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.

Expression

RNA: detected in 16 tissues
Protein: detected in 39 cell types

RNA: detected in 16 tissues
Protein: detected in 54 cell types

RNA: detected in 16 tissues
Protein: detected in 28 cell types

Retrieval

HIER pH6

HIER pH9

Antibody dilution

1:75

1:7

1:300

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006288

Antibody HPA007010

Antibody CAB000083

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

76.8, 76.3, 65.8, 65.3, 62.4, 57.6, 56.7, 55.7, 51.4, 46.6, 44.7

76.8, 76.3, 65.8, 65.3, 56.7

76.8, 76.3, 65.8, 65.3, 62.4, 57.6, 56.7, 55.7, 51.4, 46.6, 44.7, 19.6

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA006288

Antibody HPA007010

Antibody CAB000083

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA006288

Antibody HPA007010

Antibody CAB000083

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

CACPGRDRKADEDSIRKQQVSDSTKNGDAFRQNTHGIQMTSIKKRRSPDD
ELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQ

MMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLA
RLGCSSCLDYFTTQGLTTIYQIEHYS

Matching transcripts

TP63-008 - ENSP00000394337 [100%]
TP63-013 - ENSP00000389485 [100%]
TP63-001 - ENSP00000264731 [95%]
TP63-002 - ENSP00000407144 [95%]
TP63-003 - ENSP00000317510 [95%]
TP63-004 - ENSP00000346614 [95%]
TP63-009 - ENSP00000392488 [95%]
TP63-010 - ENSP00000376253 [95%]
TP63-011 - ENSP00000376256 [95%]
TP63-012 - ENSP00000387839 [95%]
TP63-015 - ENSP00000376254 [95%]

TP63-001 - ENSP00000264731 [100%]
TP63-004 - ENSP00000346614 [100%]
TP63-008 - ENSP00000394337 [100%]
TP63-012 - ENSP00000387839 [100%]
TP63-013 - ENSP00000389485 [100%]

Other gene match

ANTIGEN VIEW

TP63-001	TP63-002	TP63-003	TP63-004	TP63-008	TP63-009	TP63-010	TP63-011	TP63-012	TP63-013	TP63-015

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.